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pgl3 simian virus 40 (sv40) luc  (Promega)

 
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    Structured Review

    Promega pgl3 simian virus 40 (sv40) luc
    Pgl3 Simian Virus 40 (Sv40) Luc, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+simian+virus+40+luc/pgl3+basic/10__1128_slash_mcb__26__5__1917___1931__2006-62-22-28
    Average 90 stars, based on 1 article reviews
    pgl3 simian virus 40 (sv40) luc - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Construct:

    Article Title: RBP1 Family Proteins Exhibit SUMOylation-Dependent Transcriptional Repression and Induce Cell Growth Inhibition Reminiscent of Senescence
    Article Snippet: The truncations used in transcriptional repression assays were constructed by subcloning the various regions into pSG424 vector using the appropriate restriction endonucleases. pGL3 simian virus 40 (SV40) luc (Promega) expressing the firefly luciferase reporter gene under the control of the SV40 promoter was modified by insertion of five Gal4 DNA binding consensus sites (G5) from pG5luc (Promega) to generate pGL3 G5 SV40 luc. pG5 TK luc (herpes simplex virus minimal thymidine kinase [HSV TK] promoter) has been described previously ( 27 ). pG5 MLP luc (adenovirus major late promoter) was obtained from Promega (pG5luc).

    Subcloning:

    Article Title: RBP1 Family Proteins Exhibit SUMOylation-Dependent Transcriptional Repression and Induce Cell Growth Inhibition Reminiscent of Senescence
    Article Snippet: The truncations used in transcriptional repression assays were constructed by subcloning the various regions into pSG424 vector using the appropriate restriction endonucleases. pGL3 simian virus 40 (SV40) luc (Promega) expressing the firefly luciferase reporter gene under the control of the SV40 promoter was modified by insertion of five Gal4 DNA binding consensus sites (G5) from pG5luc (Promega) to generate pGL3 G5 SV40 luc. pG5 TK luc (herpes simplex virus minimal thymidine kinase [HSV TK] promoter) has been described previously ( 27 ). pG5 MLP luc (adenovirus major late promoter) was obtained from Promega (pG5luc).

    Plasmid Preparation:

    Article Title: RBP1 Family Proteins Exhibit SUMOylation-Dependent Transcriptional Repression and Induce Cell Growth Inhibition Reminiscent of Senescence
    Article Snippet: The truncations used in transcriptional repression assays were constructed by subcloning the various regions into pSG424 vector using the appropriate restriction endonucleases. pGL3 simian virus 40 (SV40) luc (Promega) expressing the firefly luciferase reporter gene under the control of the SV40 promoter was modified by insertion of five Gal4 DNA binding consensus sites (G5) from pG5luc (Promega) to generate pGL3 G5 SV40 luc. pG5 TK luc (herpes simplex virus minimal thymidine kinase [HSV TK] promoter) has been described previously ( 27 ). pG5 MLP luc (adenovirus major late promoter) was obtained from Promega (pG5luc).

    Virus:

    Article Title: RBP1 Family Proteins Exhibit SUMOylation-Dependent Transcriptional Repression and Induce Cell Growth Inhibition Reminiscent of Senescence
    Article Snippet: The truncations used in transcriptional repression assays were constructed by subcloning the various regions into pSG424 vector using the appropriate restriction endonucleases. pGL3 simian virus 40 (SV40) luc (Promega) expressing the firefly luciferase reporter gene under the control of the SV40 promoter was modified by insertion of five Gal4 DNA binding consensus sites (G5) from pG5luc (Promega) to generate pGL3 G5 SV40 luc. pG5 TK luc (herpes simplex virus minimal thymidine kinase [HSV TK] promoter) has been described previously ( 27 ). pG5 MLP luc (adenovirus major late promoter) was obtained from Promega (pG5luc).

    Expressing:

    Article Title: RBP1 Family Proteins Exhibit SUMOylation-Dependent Transcriptional Repression and Induce Cell Growth Inhibition Reminiscent of Senescence
    Article Snippet: The truncations used in transcriptional repression assays were constructed by subcloning the various regions into pSG424 vector using the appropriate restriction endonucleases. pGL3 simian virus 40 (SV40) luc (Promega) expressing the firefly luciferase reporter gene under the control of the SV40 promoter was modified by insertion of five Gal4 DNA binding consensus sites (G5) from pG5luc (Promega) to generate pGL3 G5 SV40 luc. pG5 TK luc (herpes simplex virus minimal thymidine kinase [HSV TK] promoter) has been described previously ( 27 ). pG5 MLP luc (adenovirus major late promoter) was obtained from Promega (pG5luc).

    Luciferase:

    Article Title: RBP1 Family Proteins Exhibit SUMOylation-Dependent Transcriptional Repression and Induce Cell Growth Inhibition Reminiscent of Senescence
    Article Snippet: The truncations used in transcriptional repression assays were constructed by subcloning the various regions into pSG424 vector using the appropriate restriction endonucleases. pGL3 simian virus 40 (SV40) luc (Promega) expressing the firefly luciferase reporter gene under the control of the SV40 promoter was modified by insertion of five Gal4 DNA binding consensus sites (G5) from pG5luc (Promega) to generate pGL3 G5 SV40 luc. pG5 TK luc (herpes simplex virus minimal thymidine kinase [HSV TK] promoter) has been described previously ( 27 ). pG5 MLP luc (adenovirus major late promoter) was obtained from Promega (pG5luc).

    Control:

    Article Title: RBP1 Family Proteins Exhibit SUMOylation-Dependent Transcriptional Repression and Induce Cell Growth Inhibition Reminiscent of Senescence
    Article Snippet: The truncations used in transcriptional repression assays were constructed by subcloning the various regions into pSG424 vector using the appropriate restriction endonucleases. pGL3 simian virus 40 (SV40) luc (Promega) expressing the firefly luciferase reporter gene under the control of the SV40 promoter was modified by insertion of five Gal4 DNA binding consensus sites (G5) from pG5luc (Promega) to generate pGL3 G5 SV40 luc. pG5 TK luc (herpes simplex virus minimal thymidine kinase [HSV TK] promoter) has been described previously ( 27 ). pG5 MLP luc (adenovirus major late promoter) was obtained from Promega (pG5luc).

    Modification:

    Article Title: RBP1 Family Proteins Exhibit SUMOylation-Dependent Transcriptional Repression and Induce Cell Growth Inhibition Reminiscent of Senescence
    Article Snippet: The truncations used in transcriptional repression assays were constructed by subcloning the various regions into pSG424 vector using the appropriate restriction endonucleases. pGL3 simian virus 40 (SV40) luc (Promega) expressing the firefly luciferase reporter gene under the control of the SV40 promoter was modified by insertion of five Gal4 DNA binding consensus sites (G5) from pG5luc (Promega) to generate pGL3 G5 SV40 luc. pG5 TK luc (herpes simplex virus minimal thymidine kinase [HSV TK] promoter) has been described previously ( 27 ). pG5 MLP luc (adenovirus major late promoter) was obtained from Promega (pG5luc).

    Binding Assay:

    Article Title: RBP1 Family Proteins Exhibit SUMOylation-Dependent Transcriptional Repression and Induce Cell Growth Inhibition Reminiscent of Senescence
    Article Snippet: The truncations used in transcriptional repression assays were constructed by subcloning the various regions into pSG424 vector using the appropriate restriction endonucleases. pGL3 simian virus 40 (SV40) luc (Promega) expressing the firefly luciferase reporter gene under the control of the SV40 promoter was modified by insertion of five Gal4 DNA binding consensus sites (G5) from pG5luc (Promega) to generate pGL3 G5 SV40 luc. pG5 TK luc (herpes simplex virus minimal thymidine kinase [HSV TK] promoter) has been described previously ( 27 ). pG5 MLP luc (adenovirus major late promoter) was obtained from Promega (pG5luc).



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